rabbit anti human vegfr2  (Cell Signaling Technology Inc)

Journal: Acta neuropathologica communications

Article Title: Development of CAR-T cell therapy for NF1/SWN-related nerve sheath tumor treatment.

doi: 10.1186/s40478-025-01965-6

Figure Lengend Snippet: Fig. 1 Expression analysis of 6 antigens in 85 benign NF1/SWN-related nerve sheath tumor samples by IHC staining. A Expression intensity of HER1, HER2, VEGFR2, B7H3, CD171 and EGFRvIII in 25 NF1 and 60 SWN-NOS tumor samples. B Typical images of HER1, HER2, VEGFR2, B7H3, CD171 and EGFRvIII expression in 3 NF1 and 6 SWN-NOS tumor samples respectively. Scale bar = 100 μm. NF1, type 1 neurofibromatosis; SWN-NOS, schwannomatosis-not otherwise specified

Article Snippet: Immunohistochemistry (IHC) was performed at ZSGBBIO (Beijing, China) on 5-μm FFPE human tissue and spheroid sections using the following unconjugated antibodies: rabbit anti-human HER1 (Abcam, ab52894), rabbit anti-human HER2 (ZSGB-BIO, ZA-0023), rabbit anti-human VEGFR2 (CST, 9698S), rabbit anti-human B7H3 (CST, #14058), rabbit anti-human CD171 (Abcam, ab208155), rabbit anti-human EGFRvIII (ZSGB-BIO, ZA-0643), rabbit anti-human TGFβ1 (Abcam, ab215715), rabbit anti-human PDL1 (Genetex, GTX104763) and rabbit anti-human S100 (ZSGB-BIO, ZA-0225).

Techniques: Expressing, Immunohistochemistry

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on VEGFR2 protein expression in rat vascular endothelial cells. ( A ) Western blot bands of indicating proteins in endothelial cells; ( B ) densitometric analysis of the ratio of the intensity of protein bands. Values are the mean ± SD of VEGFR2 expression standardized to β-actin expression in three independent experiments; ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on proliferation and migration of endothelial cells. ( A ) BrdU-positive cell rate; ( B ) migration distance statistics chart. Data expressed as mean ± SD; * p < 0.05, ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Migration

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on tube formation of endothelial cells. ( A ) Number of nodes forming a tube-like structure; ( B ) branch lengths forming the tube-like structure. Data expressed as mean ± SD; ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques:

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on the mouse matrigel plug model. ( A ) Pictures of the mouse matrigel plugs (a: PBS group, b: NC group, c: ROX group, d: sh-Vegfr2 group, and e: sh-Vegfr2 + ROX group); ( B ) hemoglobin content in matrigel plugs. Data expressed as mean ± SD; * p < 0.05, ** p < 0.01, compared with the PBS group; # p < 0.05, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques:

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on the growth and angiogenesis of/in melanoma xenografts. ( A ) Melanoma xenograft tumor volumes during the test period; ( B ) melanoma xenograft tumor weight at necropsy; ( C ) immunohistochemical expression of CD34 in melanoma xenografts. Data expressed as mean ± SD; * p < 0.05, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Immunohistochemical staining, Expressing

Journal: Respiratory research

Article Title: Altered hypoxia-induced cellular responses and inflammatory profile in lung fibroblasts from COPD patients compared to control subjects.

doi: 10.1186/s12931-024-02907-x

Figure Lengend Snippet: Fig. 1 Effect of 4 h of hypoxia exposure on gene expression levels. Genes related to remodelling: VEGFR2 (a), 5-HTR2B (b) and Collagen7 (c); cellular stress: IRE1 (d) and c-Jun (h), oxidative stress: Nrf2 (e), HIF-1α (f) and SOD3 (g) were measured by RT-qPCR in primary distal lung fibroblasts obtained from healthy subjects (n = 7) and COPD patients (n = 7) after 4 h of exposure to normoxic (21% O2) or hypoxic (1% O2) conditions. Beta-actin and 18 S were used as housekeeping genes. The data is presented as median with interquartile range. Ordinary two-way ANOVA or RM two-way ANOVA were used for unpaired and paired comparisons and the post-hoc test Fisher’s LSD was used for statistical analysis *p < 0.05, **p < 0.01

Article Snippet: The following primary antibodies were used: HIF-1α (dilution 1:50, Biotin, ab81633), HIF-2α (dilution 1:100 Novus Biologicals, Bio-Techne, #NB100132), 5HTR2B (dilution 1:300, Aviva System Biology, OAAF02801), VEGFR2 (dilution 1:200, Bio-rad, AHP1327), VEGFR3 (dilution 1:200, Abcam, GR3217142-6) and negative control (Dako, X0903), all diluted in 1% BSA in TBS.

Techniques: Gene Expression, Quantitative RT-PCR